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SEM FEI metadata scale

Overview

This tool reads FEI SEM acquistion metadata from tiff tags and set image scale based on pixel size.

Note: This macro use Bio-formats plugin to read metadata. Fiji should include the plugin in default. Otherwise, install Bio-formats manually in ImageJ.

How to use

  1. Open a “.tif” file acquired from a FEI SEM/FIB (currently acquired by Thermofisher), and make the image window active. Note that the image dimensions are not calibrated.
EMtool_04.jpg
  1. Click “SEM/FEI SEM metadata Scale”, the metadata for the image will be displayed in the log window.
EMtool_05.jpg
  1. The image dimensions are also calibrated based on the pixel size in the metadata. Scale bar can be added using the ImageJ tool AnalyzeToolsScale Bar….
EMtool_06.jpg