This tool reads FEI SEM acquisition metadata from tiff tags and set image scale based on pixel size.
Note: This macro use Bio-formats plugin to read metadata. Fiji should include the plugin in default. Otherwise, install Bio-formats manually in ImageJ.
How to use
- Open a “.tif” file acquired from a FEI SEM/FIB (currently acquired by Thermofisher), and make the image window active. Note that the image dimensions are not calibrated.
- Click “SEM/FEI SEM metadata Scale”, the metadata for the image will be displayed in the log window.
- The image dimensions are also calibrated based on the pixel size in the metadata. Scale bar can be added using the ImageJ tool Analyze › Tools › Scale Bar….