Johannes Schindelin, Francois Kusztos, Benjamin Schmid
Segmentation Editor Demo Screencast (5 min 30 sec):
Open the stack containing the structures you want to reconstruct or segment. Go the to the plugins menu and click on “Segmentation Editor”. Your stack will be embedded into a segmentation window, and another window opens, which will contain your labels later.
On the left side of the segmentation window, you see a list of available materials. You can extend and modify these materials by right-clicking into the list. For a first start, right-click on Interior, select “Rename” from the popup menu and specify the name of the label you want.
Now go through the slices and use ImageJ’s selection tools to select the regions you want to label. You don’t need to label each slice. When consecutive slices differ only slightly, you can use the built-in interpolation function to label them.
After selecting the regions to label in each slice, click the I-Button (which is next to the Plus and Minus button). This interpolates if you didn’t make selections on each slice. Now activate the “3d” checkbox. It means that the selections of all slices will be assigned to a material, not only the selection of the current slice.
Now go to the material list, select the material which you want to assign, and click on the Plus button. That’s it.
After finishing, click Ok to close the segmentation window. The labels will remain open. You must store them yourself.
Have also a look at our 3D viewer if you want to see your result in 3D.
Starting from a macro
The segmentation editor can now be started from a macro, with a user-defined set of materials. Such a macro would look like this:
// create a new Segmentation Editor call("Segmentation_Editor.newSegmentationEditor"); // Reset the material list call("Segmentation_Editor.newMaterials"); // Add a desired materials call("Segmentation_Editor.addMaterial", "MyMaterial1", 255, 255, 0); call("Segmentation_Editor.addMaterial", "MyMaterial2", 255, 0, 0);