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IsletJ is a plugin for analysis of microscopy images of Langerhans islets in ImageJ. It provides free, open source tool for analysis of islets diameter and number and for estimation of sample purity (ratio of endocrine/exocrine tissue area).


IsletJ was designed to work with plain ImageJ. It requires Java-8.

The simplest way to install the plugin is using Fiji:

  • Download current version of Fiji from here
  • Launch Fiji and run HelpUpdate…
  • Select Manage update sites
  • Check the IsletJ box and then select Close
  • Select Apply changes
  • Restart Fiji


User documentation in PDF format is available at github