Activate the Intensity Profile Tools update site in Fiji.
After installation, you get a new entry Intensity Profile Tools in the
>> menu of the ImageJ toolbar.
Clicking this menu will load 2 macro tools in the toolbar.
X/Y intensity profile panel tools When clicked, this tool will create 2 intensity profile windows : one for the vertical and one for the horizontal profile of the active image.
X/Y Line Profile Tool This is an interactive tool : when selected, hovering the mouse over an image will overlay a cross-over pattern with the vertical and horizontal line profile for the current X/Y mouse position.
Double-clicking the icon allows setting the scale factor, to adjust the amplitude of the line plot.
Original version of the code was contributed by Nicolás De Francesco and Jerome Mutterer.
See related forum post.