This plugin contains three different methods for correcting the intensity decay due to photobleaching. They all work with either 2D or 3D time series. In case of 3D time series, image properties should be appropriately set. If you are not sure, check your image header by [Image → Properties].
- Simple Ratio Method:
- Plugin version of Jens Rietdorf’s macro, extended further with 3D time series
- Exponential Fitting Method:
- Histogram Matching Method:
- A brand-new method for bleach correction.
- This algorithm first samples the histogram of initial frame, and for the successive frames, histograms are matched to the first frame. This avoids the increase in noise in the latter part of the sequence which is a problem in the above two methods.
- This method does much better restoration of bleaching sequence for segmentation but not appropriate for intensity quantification.
- See the blog entry, a bit more detail on this issue
See here: Youtube