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Colocalization Threshold

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DEPRECATION NOTICE: This page describes the Colocalization Threshold plugin, which is no longer supported or maintained. Please use Coloc 2 instead.

File

Colocalization.jar

Source

on GitHub, modified from MBF ImageJ


Purpose

Sets the thresholds for colocalization analysis, and also calculates Manders coefficients, 2D histogram / scatter plot, and other stats.

Documentation

See the great documentation for this plugin at Colocalization Analysis#Colocalization_Threshold. The method is that implemented in Costes et al. This uses a Pearson’s correlation above and below the thresholds to iteratively find where the correlation between the two images is 0 and sets the thresholds there. It is pretty robust and importantly is reproducible and non subjective.

Use of this plugin should be followed by use of the Colocalization Test plugin, to see of the results are statistically significant and better then random overlap of the signal in the images.

See also the Colocalization Analysis tutorial.