[ImageJ-devel] Generate an image using imagej-ops
Curtis Rueden
ctrueden at wisc.edu
Fri Aug 21 11:06:40 CDT 2015
Hi all,
The link Brian sent was to a topic branch, which is now merged to master.
Here is a stable permalink for it:
https://github.com/imagej/imagej-tutorials/blob/4161510aa78d7f41d10a5c440ad704da05f1ea71/jython/CreateAndConvolvePoints.py
Be aware that the tutorials repository will undergo heavy shuffling around
and additions in the next couple of weeks, as we prepare the Scripting with
Ops workshop.
Regards,
Curtis
On Wed, Aug 19, 2015 at 9:23 AM, Jan Eglinger <jan.eglinger at gmail.com>
wrote:
> Hi Brian,
>
> thanks a lot for the example script, that's a great starting point!
>
> Cheers,
> Jan
>
>
>
> On 19.08.2015 15:52, Brian Northan wrote:
>
>> Hi Jan
>>
>> I am not sure of the best to draw points that are at a sub-resolution
>> position.
>>
>> Below is an example that shows how to use imglib2 (in a script) to draw
>> points and then convolve them. (note it works with imagej-ops-0.16.0
>> and above)
>>
>>
>> https://github.com/imagej/imagej-tutorials/blob/scripting/jython/CreateAndConvolvePoints.py
>>
>> If you know the equation you want to use for a point at sub-resolution
>> maybe you can implement it using the same imglib2 interfaces...
>>
>>
>>
>>
>>
>> On Wed, Aug 19, 2015 at 7:32 AM, Jan Eglinger <jan.eglinger at gmail.com
>> <mailto:jan.eglinger at gmail.com>> wrote:
>>
>> Dear all,
>>
>> I'd like to use ImageJ-Ops to create an image with "virtual beads"
>> of a given diameter with a given intensity.
>> I thought of using a 3D Gaussian to get the spatial intensities for
>> each bead (at a random subpixel position), and then summing up
>> single bead images to get a volume with randomly distributed spots.
>> Finally, I might want to convolve these ideal 3D Gaussian spots with
>> a given point-spread function (PSF).
>>
>> I guess I'll need
>> ij.op().image().equation()
>> ij.op().math().add()
>> and finally
>> ij.op().filter().convolve()
>>
>> but I am unsure how to proceed.
>>
>> Could anybody give me some hints how to start?
>>
>> Thanks,
>> Jan
>>
>>
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