[ImageJ-devel] Generate an image using imagej-ops

[Jan Eglinger]

Hi Brian,

thanks a lot for the example script, that's a great starting point!

Cheers,
Jan



On 19.08.2015 15:52, Brian Northan wrote:
> Hi Jan
>
> I am not sure of the best to draw points that are at a sub-resolution
> position.
>
> Below is an example that shows how to use imglib2 (in a script) to draw
> points and then convolve them.   (note it works with imagej-ops-0.16.0
> and above)
>
> https://github.com/imagej/imagej-tutorials/blob/scripting/jython/CreateAndConvolvePoints.py
>
> If you know the equation you want to use for a point at sub-resolution
> maybe you can implement it using the same imglib2 interfaces...
>
>
>
>
>
> On Wed, Aug 19, 2015 at 7:32 AM, Jan Eglinger <jan.eglinger at gmail.com
> <mailto:jan.eglinger at gmail.com>> wrote:
>
>     Dear all,
>
>     I'd like to use ImageJ-Ops to create an image with "virtual beads"
>     of a given diameter with a given intensity.
>     I thought of using a 3D Gaussian to get the spatial intensities for
>     each bead (at a random subpixel position), and then summing up
>     single bead images to get a volume with randomly distributed spots.
>     Finally, I might want to convolve these ideal 3D Gaussian spots with
>     a given point-spread function (PSF).
>
>     I guess I'll need
>        ij.op().image().equation()
>        ij.op().math().add()
>     and finally
>        ij.op().filter().convolve()
>
>     but I am unsure how to proceed.
>
>     Could anybody give me some hints how to start?
>
>     Thanks,
>     Jan
>