[ImageJ-devel] Generate an image using imagej-ops

[Brian Northan]

Hi Jan

I am not sure of the best to draw points that are at a sub-resolution
position.

Below is an example that shows how to use imglib2 (in a script) to draw
points and then convolve them.   (note it works with imagej-ops-0.16.0 and
above)

https://github.com/imagej/imagej-tutorials/blob/scripting/jython/CreateAndConvolvePoints.py

If you know the equation you want to use for a point at sub-resolution
maybe you can implement it using the same imglib2 interfaces...





On Wed, Aug 19, 2015 at 7:32 AM, Jan Eglinger <jan.eglinger at gmail.com>
wrote:

> Dear all,
>
> I'd like to use ImageJ-Ops to create an image with "virtual beads" of a
> given diameter with a given intensity.
> I thought of using a 3D Gaussian to get the spatial intensities for each
> bead (at a random subpixel position), and then summing up single bead
> images to get a volume with randomly distributed spots.
> Finally, I might want to convolve these ideal 3D Gaussian spots with a
> given point-spread function (PSF).
>
> I guess I'll need
>   ij.op().image().equation()
>   ij.op().math().add()
> and finally
>   ij.op().filter().convolve()
>
> but I am unsure how to proceed.
>
> Could anybody give me some hints how to start?
>
> Thanks,
> Jan
>
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