[ImageJ-bugs] [Bug 1160] Threshold value in Trackmate is not stricktly used while tracking particles. most of the time particles considered in tracking analysis have intensity values much lower than the threshold value set before starting the analysis.

[bugzilla at fiji.sc]

http://fiji.sc/bugzilla/show_bug.cgi?id=1160

Jean-Yves Tinevez <jeanyves.tinevez at gmail.com> changed:

           What    |Removed                     |Added
----------------------------------------------------------------------------
             Status|REOPENED                    |RESOLVED
         Resolution|---                         |FIXED

--- Comment #7 from Jean-Yves Tinevez <jeanyves.tinevez at gmail.com> ---
Hi Deepika

Thank you I received the file and could play with it.

There is a major misunderstanding here, and it is caused by outdated
documentation. 

Indeed, the threshold you set in the LoG detector panel is NOT a threshold on
spot maximal intensity, but on spot QUALITY. The spot quality is a value
generated by detectors that has no direct link with maximal intensity. It
simple reflects how certain is the detector that the spot it finds is a good
one. 

However, I read the documentation again, and it states that the threshold
indeed acts on maximal intensity, which is wrong. See 
http://fiji.sc/Getting_started_with_TrackMate

I will fix this. 

Now if you want to filter spot on maximal intensity, you have to use a filter
on this value in the spot filter panel.

Thank you very much.

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