[ImageJ-bugs] [Bug 1160] Threshold value in Trackmate is not stricktly used while tracking particles. most of the time particles considered in tracking analysis have intensity values much lower than the threshold value set before starting the analysis.

[bugzilla at fiji.sc]

http://fiji.sc/bugzilla/show_bug.cgi?id=1160

--- Comment #6 from Deepika Sharma <deepika.sharma at unibas.ch> ---
Hi Jean,

Please find the file :
http://dl.pasteur.fr/fop/vYsEyEDz/09.09.2015_60XOil_30uM_Impb_SYbeads_20fps_20ms_20instensity_561nm_14-1.tif

Please use following values at consecutive steps for TrackMate based particle
analysis:
1- detector : LoG detector
2- blob diameter: 1um; Threshold: 65000.0
3- Select View: HyperStack Displayer
4- Set filters on spots: Set color by = Maximal intensity 
5- LAP Tracker 
6- Frame to frame linking: Max distance = 1.0 um (other options are unchecked)
7- Set filters on tracks: Track index
8- zoom in the track and observe the circle around the moving particle, you
will see not all particle has intensity = 65000. 

In my case I need only particle with intensity equal or higher than the
threshold values. So it would be great if you could tell me some way of
achieving that from Trackmate.

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