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Sprout Morphology
| Sprout Morphology (Fiji) | |
|---|---|
| Author | Jan Eglinger |
| Maintainer | Jan Eglinger |
| Source | on GitHub |
| Development status | stable |
| Category | Analysis |
The Sprout Morphology plugin measures sprout number, length, width and cell density of endothelial cell (EC) sprouts grown in a bead sprouting assay. It optionally includes measuring the coverage of these sprouts with pericytes included in the assay, as well as the endothelial cell/pericyte ratio.
Contents
Installation
To install the plugin, activate the Angiogenesis update site and restart Fiji.
Usage
Open a maximum intensity projection of the multi-channel image to be analyzed, then start the plugin via Analyze › Sprout Morphology. The process consists of up to six dialogs:
General configuration
Bead detection
Sprout detection
Nucleus segmentation
Endothelial cell/Pericyte classification
Pericyte coverage
Publication
- Eglinger, J.; Karsjens, H. & Lammert, E. (2017), "Quantitative assessment of angiogenesis and pericyte coverage in human cell-derived vascular sprouts", Inflammation and Regeneration 37(2), doi:10.1186/s41232-016-0033-2, <http://inflammregen.biomedcentral.com/articles/10.1186/s41232-016-0033-2>
