This is an archive of the old MediaWiki-based ImageJ wiki. The current website can be found at


Project SCIFIO
Source on GitHub
License BSD-2
Release 0.32.0
Date Wed May 24 10:17:58 CDT 2017
Development status Active
Support status Active
Founders Curtis Rueden, Mark Hiner
Leads Curtis Rueden
Developers Curtis Rueden
Debuggers Curtis Rueden
Reviewers Curtis Rueden
Support Curtis Rueden
Maintainers Curtis Rueden
Contributors Mark Hiner, Johannes Schindelin, Andrea Ballaminut, Gabriel Einsdorf, Henry Pinkard, Ray Plante, Daniel Seebacher, Richard Domander

SCIFIO is a flexible framework for SCientific Image Format Input and Output. In other words, it is a library for reading and writing N-dimensional image data—e.g., to and from files on disk. SCIFIO is built on the SciJava Common library.

ImageJ2 uses SCIFIO by default for most image input tasks. You can change this behavior at any time by running Edit  › Options  › ImageJ2 and modifying the Use SCIFIO when opening files option.

Benefits of using SCIFIO

SCIFIO is focused on robust and extensible support for reading and writing image file formats. Using it with ImageJ provides many advantages:

  • There is no need to call a special SCIFIO plugin; it works with commands like File  › Open automatically.
  • There are additional import options available via the File  › Import  › Image... command.
  • There is a Bio-Formats plugin for SCIFIO, included with the Fiji distribution of ImageJ, that adds automatic support for over a hundred life sciences file formats.
  • Additional SCIFIO file format plugins can be dropped into ImageJ and will also work automatically.
  • Unlike the ImageJ 1.x TIFF implementation, SCIFIO's support for TIFF adheres to the specification, allowing to successfully read many more sorts of TIFFs.
  • Similarly, SCIFIO supports more sorts of JPEG files since it uses its own JPEG decoder.
  • SCIFIO also ships with support for several QuickTime codecs, allowing ImageJ to read QuickTime MOV files even in 64-bit mode without QuickTime for Java.
  • SCIFIO supports many additional open file formats out of the box:
    • animated GIF
    • animated PNG
    • encapsulated postscript (EPS)
    • JPEG-2000
    • Micro-Manager datasets
    • Multi-image Network Graphics (MNG)
    • Nearly Raw Raster Data (NRRD)
    • Imspector OBF
    • OME-TIFF (multidimensional rich metadata TIFF)
    • OME-XML
    • PCX
    • PICT (even in 64-bit mode and/or without QuickTime for Java installed)
  • If SCIFIO cannot handle the image file, it falls back to ImageJ 1.x.
  • You can save to SCIFIO-supported file formats using the File  › Export  › Image... command. Supported formats for export include:
    • APNG
    • AVI
    • EPS
    • ICS
    • JPEG
    • JPEG2000
    • QuickTime
    • TIFF

Current limitations of SCIFIO

  • SCIFIO is still in beta, so there is likely to be a higher incidence of bugs. Issues can be reported on the SCIFIO issue tracker.
  • Although we strive for full backwards compatibility, some files may appear slightly different when opened.
  • Opening files with SCIFIO is not fully macro recordable yet.