Computer Assisted Sperm Analyzer

Computer Assisted Sperm Analyzer

Authors: Jonas Wilson-Leedy, Rolf Ingermann
Department of Biology
University of Idaho
wils0959 @ uidaho.edu, rolfi @ uidaho.edu

History: 2006/6/10: first version

Source: Included in CASA_.java, and is released under the GNU General Public License. This plugin is subject to the license agreement below.

Installation: Download CASA_.java to the plugins folder, compile and run.

Requires: Tested using ImageJ 1.36 with Java 1.3.1_13 on Windows XP pro

Description: This plugin will analyze video of sperm in the form of a stack and will output commonly reported parameters of sperm motion as well as the percentage of sperm moving.

Please review reference below as well as the PDF manual provided: CASA instructions.pdf. Please also visit the following website for further information; http://wilson-leedy.com/CASA/CASA.html.

This plugin is subject to the license agreement below.

Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters

Jonas G. Wilson-Leedy and Rolf L. Ingermann

Abstract
Although computer assisted sperm analysis (CASA) outperforms manual techniques, many investigators rely on non-automated analysis due to the high cost of commercial options. In this study, we have written and validated a free CASA software primarily for analysis of fish sperm. This software is a plugin for the free National Institutes of Health software ImageJ and is available with documentation at https://rsb.info.nih.gov/ij/plugins/casa.html. That it is open source makes possible external validation, should improve quality control and enhance the comparative value of data obtained among laboratories. In addition, we have improved upon the traditional velocity straight line (VSL) algorithm, eliminating inaccurate characterization of highly curved fish sperm paths. Using this system, the motion of zebrafish (Danio rerio) sperm was characterized relative to time post-activation and the impact of acquisition conditions upon data analysis determined. There were decreases in velocity and path straightness (STR), but not linearity (LIN), relative to time. From 30 to 300 frames/sec, frame rate significantly affected curvilinear velocity (VCL) and STR measurements. Sperm density in the field of view did not affect any measured parameter. There was significant inter-male variation for VCL, VSL, velocity average path (VAP), percent motility, path character (STR, LIN), and duration of motility. Furthermore, relative sperm output (a measure reflecting both semen volume and concentration) was positively correlated to percent motility. For all motion parameters measured (except duration), the average CV was < 10 %, comparable to values obtained using commercial systems.
Wilson-Leedy JG, Ingermann RL, Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters, Theriogenology (2006), doi:10.1016/j.theriogenology.2006.10.003.

Copyright © 2003 The Regents of the University of California and the Howard Hughes Medical Institute.

All Rights Reserved.

Permission to use, copy, modify, and distribute this software and its documentation for educational, research and non-profit purposes, without fee, and without a written agreement is hereby granted, provided that the above copyright notice, this paragraph and the following three paragraphs appear in all copies.

Permission to incorporate this software into commercial products may be obtained by contacting the Office of Technology Management at the University of California San Francisco [Sunita Rajdev, Ph.D., Licensing Officer, UCSF Office of Technology Management. 185 Berry St, Suite 4603, San Francisco, CA 94107].

This software program and documentation are copyrighted by The Regents of the University of California acting on behalf of the University of California San Francisco via its Office of Technology Management and the Howard Hughes Medical Institute (collectively, the Institution). The software program and documentation are supplied "as is", without any accompanying services from the Institution. The Institution does not warrant that the operation of the program will be uninterrupted or error-free. The end-user understands that the program was developed for research purposes and is advised not to rely exclusively on the program for any reason.

IN NO EVENT SHALL THE INSTITUTION BE LIABLE TO ANY PARTY FOR DIRECT, INDIRECT, SPECIAL, INCIDENTAL, OR CONSEQUENTIAL DAMAGES, INCLUDING LOST PROFITS, ARISING OUT OF THE USE OF THIS SOFTWARE AND ITS DOCUMENTATION, EVEN IF THE INSTITUTION HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. THE INSTITUTION SPECIFICALLY DISCLAIMS ANY WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE SOFTWARE PROVIDED HEREUNDER IS ON AN "AS IS" BASIS, AND THE INSTITUTION HAS NO OBLIGATIONS TO PROVIDE MAINTENANCE, SUPPORT, UPDATES, ENHANCEMENTS, OR MODIFICATIONS.

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Authors:	Jonas Wilson-Leedy, Rolf Ingermann Department of Biology University of Idaho wils0959 @ uidaho.edu, rolfi @ uidaho.edu
History:	2006/6/10: first version
Source:	Included in CASA_.java, and is released under the GNU General Public License. This plugin is subject to the license agreement below.
Installation:	Download CASA_.java to the plugins folder, compile and run.
Requires:	Tested using ImageJ 1.36 with Java 1.3.1_13 on Windows XP pro
Description:	This plugin will analyze video of sperm in the form of a stack and will output commonly reported parameters of sperm motion as well as the percentage of sperm moving. Please review reference below as well as the PDF manual provided: CASA instructions.pdf. Please also visit the following website for further information; http://wilson-leedy.com/CASA/CASA.html. This plugin is subject to the license agreement below. Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters Jonas G. Wilson-Leedy and Rolf L. Ingermann Abstract Although computer assisted sperm analysis (CASA) outperforms manual techniques, many investigators rely on non-automated analysis due to the high cost of commercial options. In this study, we have written and validated a free CASA software primarily for analysis of fish sperm. This software is a plugin for the free National Institutes of Health software ImageJ and is available with documentation at https://rsb.info.nih.gov/ij/plugins/casa.html. That it is open source makes possible external validation, should improve quality control and enhance the comparative value of data obtained among laboratories. In addition, we have improved upon the traditional velocity straight line (VSL) algorithm, eliminating inaccurate characterization of highly curved fish sperm paths. Using this system, the motion of zebrafish (Danio rerio) sperm was characterized relative to time post-activation and the impact of acquisition conditions upon data analysis determined. There were decreases in velocity and path straightness (STR), but not linearity (LIN), relative to time. From 30 to 300 frames/sec, frame rate significantly affected curvilinear velocity (VCL) and STR measurements. Sperm density in the field of view did not affect any measured parameter. There was significant inter-male variation for VCL, VSL, velocity average path (VAP), percent motility, path character (STR, LIN), and duration of motility. Furthermore, relative sperm output (a measure reflecting both semen volume and concentration) was positively correlated to percent motility. For all motion parameters measured (except duration), the average CV was < 10 %, comparable to values obtained using commercial systems. Wilson-Leedy JG, Ingermann RL, Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters, Theriogenology (2006), doi:10.1016/j.theriogenology.2006.10.003. Copyright © 2003 The Regents of the University of California and the Howard Hughes Medical Institute. All Rights Reserved. Permission to use, copy, modify, and distribute this software and its documentation for educational, research and non-profit purposes, without fee, and without a written agreement is hereby granted, provided that the above copyright notice, this paragraph and the following three paragraphs appear in all copies. Permission to incorporate this software into commercial products may be obtained by contacting the Office of Technology Management at the University of California San Francisco [Sunita Rajdev, Ph.D., Licensing Officer, UCSF Office of Technology Management. 185 Berry St, Suite 4603, San Francisco, CA 94107]. This software program and documentation are copyrighted by The Regents of the University of California acting on behalf of the University of California San Francisco via its Office of Technology Management and the Howard Hughes Medical Institute (collectively, the Institution). The software program and documentation are supplied "as is", without any accompanying services from the Institution. The Institution does not warrant that the operation of the program will be uninterrupted or error-free. The end-user understands that the program was developed for research purposes and is advised not to rely exclusively on the program for any reason. IN NO EVENT SHALL THE INSTITUTION BE LIABLE TO ANY PARTY FOR DIRECT, INDIRECT, SPECIAL, INCIDENTAL, OR CONSEQUENTIAL DAMAGES, INCLUDING LOST PROFITS, ARISING OUT OF THE USE OF THIS SOFTWARE AND ITS DOCUMENTATION, EVEN IF THE INSTITUTION HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. THE INSTITUTION SPECIFICALLY DISCLAIMS ANY WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE SOFTWARE PROVIDED HEREUNDER IS ON AN "AS IS" BASIS, AND THE INSTITUTION HAS NO OBLIGATIONS TO PROVIDE MAINTENANCE, SUPPORT, UPDATES, ENHANCEMENTS, OR MODIFICATIONS.