Mach Bands
To generate Mach bands, first open a blank window using the File - New command. Then select the entire window using Edit - Select All , and fill it with a gray level ramp using Edit - Draw Scale . After setting the number of gray levels to a number, such as 6, with Options - LUT Options , the ramp will appear as a series of stripes, which appear slightly shaded like Venetian Blinds (Fig. 16). The bars are actually uniform, as can be seen by covering up all but one bar, by using the line profile tool, by inspecting the Info window while moving the cursor over the bars, or by doing a mesh plot using Analyze - Surface Plot . Notice that it is necessary to use Process - Apply LUT before profile plots and mesh plots show the bands. The Mach bands are caused by the abrupt intensity change at the edges, which the eye further enhances.
 
Often, annotated gray scale wedges or color bars are  used to infer numerical values 
from images using the gray  scales or colors.  In some instances, in particular when 
a  more natural or smooth color scale is used, this can be  prone to error.  
  To show the illusion, fill a window with a gray  level ramp as with the Mach Band 
illusion. If the 'crawling  ants' border is still there, get rid of it either with 
Edit  - Deselect All
 or by clicking on the image with any tool   except the four to the upper right.  
Then select the  rectangular selection tool, and click and drag it to create  a small 
rectangular selection.  Copy the selection and paste  it back where it was using 
Edit - Copy
  and Edit - Paste
.
 The selection can now be moved about the window by  dragging it with the mouse - 
do not click outside this  region until you are finished.  The selection will appear 
to  change in brightness as it is moved across the ramp.  If it  is pasted in various 
places on the ramp, it will appear to  have different gray levels, which in fact it 
does not.   (Fig. 17).  The illusion persists when using natural color  scales such 
as the thermal scale (Fig. 18) which is applied  using the Options - Color Tables - Fire 1
 command.  The  illusion does not persist, however,  when using color scales  such 
as the spectrum color scale (Fig. 19), applied using  the Options - Color Tables - Spectrum 
command that the eye  perceives as 'op art' rather than as a shaded image. 
If our perception of gray levels is faulty here, then our perception is also likely to be faulty when 'matching' gray levels in an image to a gray ramp or color bar.
 
 
