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 Sec. 11: Overlays Up Part II: Working with IJ Sec. 13: Settings & Preferences 

12 3D Volumes

Currently, the support for 3D ROIs (selections containing contiguous cluster of voxels) is somewhat limited in ImageJ. This limitation has been addressed by ImageJ2↑ and several IJ1 plugins. The list below summarizes some of the ImageJ plugins that deal effectively with multi-dimensional objects. Note that a manual installation of these tools as standalone ImageJ plugins is a challenging task given their special dependencies, reason why they are all bundled as part of Fiji↑.
figure images/3Dviewer.png
Figure 9 3D Viewer (Fiji 1.46o), bringing hardware-accelerated 3D visualization to ImageJ. As explained in 3D Volumes↑, most of plugins that truly extend ImageJ functionally to multi-dimentional data are bundled as part of Fiji.
3D Filters Specialized 3D filters such as Process▷Filters▷Gaussian Blur 3D…↓ can be installed to perform 3D operations. Examples are the 3D processing package by Thomas Boudier [31] and the 3D binary filters by Benjamin Schmid.
3D Object Counter 3D Object Counter (3D-OC) counts and qualifies 3D objects in a stack [87], similarly to the 2D analysis performed by Analyze▷Analyze Particles…↓ It is complemented by 3D Roi Manager [31], a companion plugin that adds a 3D ROI Manager↓ to ImageJ
3D Viewer 3D Viewer brings powerful hardware-accelerated 3D visualization to ImageJ [13], extending the limited functionality of Image▷Stacks▷3D Project…↓ In the ImageJ 3D Viewer↑ stacks can be displayed as texture-based volume renderings, surfaces or orthoslices. It is macro-recordable and can be used by other plugins as a high-level programming library for 3D visualization
Simple Neurite Tracer Simple Neurite Tracer allows semi-automated segmentation of tubular structures in 3D [71]
TrakEM2 As mentioned earlier, TrakEM2↑ features powerful tools for multi-dimensional regions of interest [6]
 Sec. 11: Overlays Up Part II: Working with IJ Sec. 13: Settings & Preferences