[ImageJ-bugs] [Bug 1109] New: Grid/Collection stitching not reading Prairie/Bruker stage location metadata
bugzilla at fiji.sc
bugzilla at fiji.sc
Tue Jul 7 10:21:12 CDT 2015
http://fiji.sc/bugzilla/show_bug.cgi?id=1109
Bug ID: 1109
Summary: Grid/Collection stitching not reading Prairie/Bruker
stage location metadata
Product: Fiji
Version: unspecified
Hardware: PC
OS: Windows
Status: NEW
Severity: normal
Priority: P5
Component: Plugins
Assignee: imagej-bugs at imagej.net
Reporter: thex1000 at gmail.com
Created attachment 253
--> http://fiji.sc/bugzilla/attachment.cgi?id=253&action=edit
Sample Bruker Montage data set
See attached sample Bruker data set.
1. In Fiji: Plugins-> Stitching -> Grid/Collection Stitching
2. Type: Positions from file. Order: Defined by image metadata.
3. Unzip data set.
Settings for Grid Stitching
Multi series file: Choose AtlasVolume-07072015-0914-008.xml
Invert X coordinates checked
Invert Y coordinates unchecked
Compute overlap unchecked. This forces the plugin to read in coordinates.
4. Click OK.
In the log window, the positions are read in as 0.0 instead of the actual stage
locations and the resulting stitched image is incorrect.
Other settings that do not matter as much:
Fusion method: linear Blending
Regression th: 0.3
Max/avg displacement th: 2.50
Absolute displacement th: 3.50
Add tiles as ROIs unchecked
Ignore calibration unchecked
Invert z stage position unchecked
subpixel accuracy checked
downsample tiles unchecked
display fusion unchecked
use virtual images unchecked
computation parameters: save memory
image output: fuse and display
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