[ImageJ-bugs] [Bug 1053] New: Some DeltaVision (.dv) files are read incorrectly

bugzilla at fiji.sc bugzilla at fiji.sc
Fri Apr 10 15:24:14 CDT 2015


http://fiji.sc/bugzilla/show_bug.cgi?id=1053

            Bug ID: 1053
           Summary: Some DeltaVision (.dv) files are read incorrectly
           Product: Fiji
           Version: unspecified
          Hardware: PC
                OS: Mac OS
            Status: NEW
          Severity: major
          Priority: P5
         Component: Bio-Formats
          Assignee: imagej-bugs at imagej.net
          Reporter: jcf14 at cornell.edu

Some DeltaVision files (for example, see attached uploaded image
"2269_37C_12_R3D.dv" are read into FIJI in a way that the intensities are on a
scale of -32728 to 32767 (instead of 0 to 65535). This then leads to a problem
when trying to quantitate intensity values, for example, using multi-measure
through the ROI manager. This happens on both a Mac OS X and Windows 8 machine.
As it does not happen with all DeltaVision files, it may be an issue with how
DeltaVision is writing the files, but I don't know why that would be.

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