// Three Color Overlay Plot of a Line run("Fluorescent Cells (400K)"); if (!is("composite")) run("Make Composite"); id = getImageID; setBatchMode(true); run("Profile Plot Options...", "width=450 height=200 minimum=0 maximum=255 fixed interpolate draw"); makeLine(50, 50, getWidth-50, getHeight-50); run("Plot Profile"); run("Invert"); rename("red"); selectImage(id); setSlice(2); run("Plot Profile"); run("Invert"); rename("green"); selectImage(id); setSlice(3); run("Plot Profile"); run("Invert"); rename("blue"); run("Merge Channels...", "red=red green=green blue=blue gray=*None* create"); setBatchMode(false);