// "SubtractMeasuredBackground" // For each image in a stack, this macro subtracts the // background level calculated from the current selection. // Note that the selection may be a composite (discontinuous) // selection that was created by holding down the shift key. macro "Subtract Measured Background 1" { if (selectionType==-1) exit("This macro requires an area selection"); for (i=1; i<=nSlices; i++) { setSlice(i); getStatistics(area, mean); run("Select None"); run("Subtract...", "value="+mean); run("Restore Selection"); } } // This macro subtracts the mean gray level calculated // from user-defined selections that have been added to the // ROI Manager. It works with both single images and a stacks. macro "Subtract Measured Background 2" { n = roiManager("count"); if (n==0) exit("This macro requires at least one ROI Manager entry"); sum = 0; for (i=0; i