|Maintainer||Albert Cardona (firstname.lastname@example.org)|
|File||TrakEM2_.jar (Included in Fiji)|
|Source||trakem2 git repository|
|Initial release||May 2006|
|Latest version||0.7m (4 December 2009)|
|Category||Registration, Segmentation, Image annotation, Plugins|
|Website||TrakEM2 news and documentation|
TrakEM2 is an ImageJ plugin for morphological data mining, three-dimensional modeling and image stitching, registration, editing and annotation.
See TrakEM2 snapshots for an overview.
- Segmentation: manually draw areas across stacks, and sketch structures with balls and pipes.
- Measurements: volumes, surfaces, lengths, and also measurements via ImageJ ROIs.
- Image Registration: register floating image tiles to each other using SIFT and global optimization algorithms.
- 3D Visualization: interacting with the 3D Viewer plugin, TrakEM2 displays image volumes and 3D meshes of all kinds.
- Image Annotation: floating text labels.
- Semantic segmentation: order segmentations in tree hierarchies, whose template is exportable for reuse in other, comparable projects.
TrakEM2 interacts with the 3D Viewer for visualization of image volumes and 3D meshes.
TrakEM2 in Fiji
- Create new projects from "File - New - TrakEM2 (blank)"
- Open an existing project by dragging its .xml file onto the toolbar, or via "File - Open".
Running fiji for heavy-duty, memory-intensive, high-performance TrakEM2 tasks
The following configuration has been tested in a machine with 8 CPU cores and 16 Gb of RAM, running Ubuntu 8.04 "Hardy":
./fiji -Xms10g -Xmx10g -Xincgc -XX:MaxPermSize=512m -XX:PermSize=512m -XX:NewRatio=5 -XX:CMSTriggerRatio=50 -XX:+UseCompressedOops --
Put all the above in one line.
What the JVM flags mean:
- -Xms10g : use an initial heap size of 10 Gb (i.e. start fiji with 10 Gb of RAM preallocated to it)
- -Xmx10g: use a maximum heap size of 10 Gb. Note it's the same amount as the intial heap size, so that the heap cannot be resized.
- -Xincgc : use the incremental garbage collector. Clean up unused memory in the concurrent mark-sweep generation (i.e. not the super-new generation of allocated objects) using parallel threads.
- -XX:MaxPermSize=512m : set the heap size allocated to objects that don't need to be garbage often to half a gygabyte. The default is only 32m, which proves insufficient.
- -XX:PermSize=512m : preallocate the heap for the permanent objects directly to its maximum, half a gygabyte, so it doesn't ever have to be resized.
- XX:NewRatio=5 : Set the ratio of ephemeral versus more long-lived objects to 5 (the default is 9 or more in 64-bit Sun JVMs).
- XX:CMSTriggerRatio=50 : run the parallel garbage collector when the ratio of free versus non-free heap space is 50 % (the default is 92% in 64-bit JVM, which may incur in pauses and undesirable full sweeps).
- XX:+UseCompressedOops : use 32-bit pointers when possible, in a 64-bit JVM. This can cut the memory footprint by half in many cases.
- The ending double hyphen "--" is to specify all of these are JVM arguments, not fiji/ImageJ arguments.
With the above settings, we have succesfully registered 77,000 image tiles corresponding to 459 serial sections, using the "Align multi-layer mosaic" TrakEM2 command.