The problem with tracking algorithms is that they always give an answer.
This answer can be completely irrelevant, even non-physical, and there is no built-in flags that would indicate something wrong. The best way to avoid basing your downstream analysis on faulty tracking results is to know in what situation the tracker works the best, and what are its limitations. This is the aim of this page for the trackers and detectors shipped with TrackMate.
The ISBI 2012 single particle challenge.
In 2011-2012, an ISBI Grand Challenge was organized for the Single-Particle Tracking algorithms. Though TrackMate does not offer a completely new algorithm, product of an original Research work, we took the chance and participated in the challenge. The results and the methodology to compute the accuracy of a tracking algorithms were published thereafter.
Unsurprisingly, we did not score amongst the best. At the time, TrackMate was in version 1.1, and ship a stripped down version of the better performing Jaqaman et al. LAP framework. See the LAP trackers section for algorithm details.