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== Simple Neurite Tracer: Accurate point placement == Note that there were recent changes that made the use of the plugin in three pane mode much easier, so make sure that you have an up-to-date Fiji by running "Help > Update Fiji". === Summary === Sometimes people have reported problems with placing points accurately. Many of these issues can be resolved just by placing the points when: * Using the "Three pane view"* Zoomed in to 600% or so A step by step guide is given below: === Step-by-step === When starting up, make sure that "Three pane view" is turned on: [[Image:Simple_Neurite_Tracer_Accurate_point_placement-1.png]] Find the approximate location of your start point by moving your mouse in the XY window, holding down Shift to synchronize the view in the other panes: [[Image:Simple_Neurite_Tracer_Accurate_point_placement-2.png]] When you press "+" to zoom in, all the panes will zoom in on the point that the crosshairs is over, so each time you press "+", make sure you move your mouse pointer so that it's still over the structure of interest: [[Image:Simple_Neurite_Tracer_Accurate_point_placement-3.png]] Then click to start the path: #REDIRECT [[ImageSNT:Simple_Neurite_Tracer_Accurate_point_placement_Step-4.png]] Zoom out again with the "By-" key, and similarly zoom in on the next point you want to be on your path to place it precisely: [[Image:Simple_Neurite_Tracer_Accurate_point_placement-5.png]] The path along the neuron may not follow the centre line: [[Image:Simple_Neurite_Tracer_Accurate_point_placement-6.png]] ... but you can later improve that with the "Fit Volume" option, which tries to fix the midline and radius to sub-voxel accuracy: [[Image:Simple_Neurite_Tracer_Accurate_point_placement-7.png]]   [[Category:TutorialsStep_Instructions#Accurate_Point_Placement]]
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