Simple Neurite Tracer: Accurate point placement
Note that there were recent changes that made the use of the plugin in three pane mode much easier, so make sure that you have an up-to-date Fiji by running "Help > Update Fiji".
Sometimes people have reported problems with placing points accurately. Many of these issues can be resolved just by placing the points when:
- Using the "Three pane view"
- Zoomed in to 600% or so
A step by step guide is given below:
When starting up, make sure that "Three pane view" is turned on:
Find the approximate location of your start point by moving your mouse in the XY window, holding down Shift to synchronize the view in the other panes:
When you press "+" to zoom in, all the panes will zoom in on the point that the crosshairs is over, so each time you press "+", make sure you move your mouse pointer so that it's still over the structure of interest:
Then click to start the path:
Zoom out again with the "-" key, and similarly zoom in on the next point you want to be on your path to place it precisely:
The path along the neuron may not follow the centre line:
... but you can later improve that with the "Fit Volume" option, which tries to fix the midline and radius to sub-voxel accuracy: