What is metadata?
When acquiring images in microscopy, the image files that are stored contain two main things:
- image data, which is essentially 'pixel values'
- metadata, information on the image data including pixel size, bit depth, dimension and objective information, etc.
Metadata is essential to correctly read image data; for example, to have accurate measurements, the image needs to be calibrated according to the correct/associated pixel size. Saving and preserving metadata is key in quantitative image analysis.