- 1 The Jython interpreter plugin
- 2 Jython tutorials for ImageJ
The Jython interpreter plugin
The interpreter provides a screen and a prompt. Type any jython code on the prompt to interact with ImageJ.
Within the interpreter, all ImageJ, java.lang.* and TrakEM2 classes are automatically imported. So creating new images and manipulating them is very straighforward.
- Any text after a # is commented out.
- There are no line terminators (such as ';' in other languages), neither curly braces to define code blocks.
- Indentation defines code blocks.
- Functions are defined with def, and classes with class.
- Functions are objects, and thus storable in variables.
- Jython (and python in general) accepts a mixture of procedural and object-oriented code.
- Jython currently implements the Python language at its 2.5 version. All documentation for python 2.5 applies to Jython bundled with Fiji (with the remarks listed later).
Workflow for creating Jython scripts
The recommended setup is the following:
- Edit a file in your favorite text editor, and save it with a an underscore in the name and a .py extension anywhere under ImageJ plugins folder.
- Run Plugins - Scripting - Refresh Jython scripts only the very first time after newly creating the file under any folder or subfolder of ImageJ's plugins folder. A menu item will appear with its name, from which it can be run.
- Keep editing (and saving) the file from your editor. Just select the menu item to execute it over and over. Or use the "Find..." command window to launch it easily (keybinding 'l').
The next time Fiji is run, automatic commands in macros/StartupMacros.txt will setup all your scripts in the Plugins menu.
Some limitations of jython
Though jython tries to be as close as possible as python, there are some differences you may experience during scripting.
- Float "special numbers" such as NaN and Inf are not handled.
a = float('nan')
will create the correct float number in python, but will throw an exception in jython.
Instead, to create a NaN in jython, use:
>>> a = Double.NaN >>> print a NaN
To test if a number is NaN:
>>> if Double.isNaN(a): print "a is NaN!" a is NaN!
- Some existing python modules can't be imported in jython.
This is for instance the case of the module numpy, which would have been really convenient for analysing data ad results.
But see these java numerical libraries: http://math.nist.gov/javanumerics/#libraries , of which:
- JaMa (Java Matrix Package)
- Java3D (particularly its vecmath package provides general matrix and vector classes (GMatrix, GVector).
... are already included in Fiji.
Jython tutorials for ImageJ
Defining variables: obtaining the current image
imp = IJ.getImage()
Which is the same as:
imp = WindowManager.getCurrentImage()
Since calling the above is long and tedious, one can declare a variable that points to the above static methods:
c = WindowManager.getCurrentImage
Above note the lack of parentheses.
To execute the function, just use parentheses on it:
imp = c()
The above gets the value of c, which is the method named getCurrentImage in class WindowManager, and executes it, storing its returned object in imp.
Creating a grayscale ramp image
First create an image and obtain its pixels:
imp = ImagePlus("my new image", FloatProcessor(512, 512)) pix = imp.getProcessor().getPixels()
The length of an array:
n_pixels = len(pix)
Then loop to modify them:
# catch width w = imp.getWidth() # create a ramp gradient from left to right for i in range(len(pix)): pix[i] = i % w # adjust min and max, since we know them imp.getProcessor().setMinAndMax(0, w-1)
... and show the new image:
Creating a random 8-bit image
First import necessary packages: Random, from standard java util library, and jarray, the Jython module for native java arrays:
from java.awt import Random from jarray import zeros
Then create the array and fill it with random bytes:
width = 512 height = 512 pix = zeros(width * height, 'b') Random().nextBytes(pix)
(See the jarray documentation where the 'b'-byte, 'd'-double, etc. are explained.)
Now make a new IndexColorModel (that's what ImageJ's ij.process.LUT class is) for 8-bit images:
channel = zeros(256, 'b') for i in range(256): channel[i] = (i -128) cm = LUT(channel, channel, channel)
... and compose a ByteProcessor from the pixels, and assign it to an ImagePlus:
imp = ImagePlus("Random", ByteProcessor(width, height, pix, cm) imp.show()
Creating a random image, the easy way
All the above can be summarized like the following:
from java.util import Random imp = IJ.createImage("A Random Image", "8-bit", 512, 512, 1) Random().nextBytes(imp.getProcessor().getPixels()) imp.show()
Running a watershed plugin on an image
# Obtain an image imp = IJ.openImage("http://rsb.info.nih.gov/ij/images/bridge.tif") ip = imp.getProcessor() # apply a threshold: only zeros and ones ip.setThreshold(0, 74) Thresholder().run("mask") ip.invert() # apply watershed edm = EDM() edm.setup("watershed", imp) edm.run(ip) imp.show()
The EDM plugin that contains the watershed could have been indirectly applied to the currently active image, which is not recommended:
If you had called show() on the image at any early stage, just update the screen with: