Difference between revisions of "FLIMJ"

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Fluorescence lifetime analysis in ImageJ.
 
 
 
The FLIMJ plugin for ImageJ provides the ability to analyze FLIM data within ImageJ, using the [https://flimlib.github.io/ FLIMLib] library. The plugin can be installed into the [[Fiji]] distribution of ImageJ simply by enabling the FLIMJ [[update site]]. Features include:
 
The FLIMJ plugin for ImageJ provides the ability to analyze FLIM data within ImageJ, using the [https://flimlib.github.io/ FLIMLib] library. The plugin can be installed into the [[Fiji]] distribution of ImageJ simply by enabling the FLIMJ [[update site]]. Features include:
  

Revision as of 12:58, 16 October 2019

The FLIMJ plugin for ImageJ provides the ability to analyze FLIM data within ImageJ, using the FLIMLib library. The plugin can be installed into the Fiji distribution of ImageJ simply by enabling the FLIMJ update site. Features include:

  • Fit individual pixels, entire images per-pixel, or do global analysis on entire images, using FLIMLib's rapid lifetime determination (RLD), Levenberg-Marquardt (LMA) or global analysis (Global) fitting algorithms
  • Single, double and triple exponential fits
  • Gaussian, Poisson and Maximum Likelihood Estimation noise models
  • Produce one or several fitted images depending which parameters (A, τ, Z, χ2) are chosen for visualization
  • Full control over the start and end fit cutoffs known as "cursors"
  • Binning options for various kernel sizes to reduce noise and boost intensity when fitting per-pixel
  • Support for so-called "excitation" or "prompt" files containing a recorded system response function to be convolved with the exponential fit
  • Batch processing support for analyzing many lifetime images as part of a scripting workflow

More documentation coming soon. For now, see the source code at:

https://github.com/slim-curve/flimj-ops