DS4H Image Alignment

Revision as of 10:36, 21 August 2019 by DS4H (talk | contribs)
DS4H Image alignment (ImageJ)
Author Stefano Belli; Antonella Carbonaro; Filippo Piccinini
Maintainer Template:DS4H
Source on GitHub
Initial release 08/06/2019
Latest version 13/08/2019
Development status stable
Category Category:Registration


"Data Science for Health (DS4H) Image Alignment" is a user-friendly tool freely provided as an ImageJ/Fiji plugin. With DS4H Image Alignment,images can be easily aligned (i.e. co-registered) by defining with a few clicks some well visible reference marks.

The implemented least-squares method automatically approximates the solution of the mathematical overdetermined system, so to define the registration matrix then used for aligning the different images. It also considers rotations and scale changes in case of object dilation/shrink. Finally, it provides an iterative subroutine for a fine alignment, to easily reach a very good image co-registration quality.

DS4H Image Alignment has been implemented in Java as a plugin for ImageJ/Fiji. It works with “.svs” files, but also all the medical imaging formats included in Bio-Formats. It is freely available at: www.filippopiccinini.it/DS4H-IA.html, together with a sample dataset and a video tutorial.

Image support

All the most common biological image formats (like .tiff, .svs, .ndpi2, etc.) are supported and openable directly from the plugin. This has been possible thanks to the use of the Bio-formats library.