Cookbook

Revision as of 08:08, 5 April 2009 by Schindelin (talk | contribs) (Add a very rough initial sketch of what might be part of the cookbook)
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Introduction

This book intends to be a users' guide for Fiji. No prior knowledge of ImageJ, programming, or "computer lingo" should be necessary to understand the recipes.

Recipes

Cooks call them recipes, biologists protocols, and programmers call them HOWTOs. No matter how you refer to them, here they are:

How to get the appropriate version of Fiji running

How to open/import raw data

Remark: programmers are often confused what you mean by raw data -- you talk about unprocessed data, while they refer to a certain file format.

Images come in many formats: LSM, Metamorph, Spot, CellR..... etc. Most of them can be opened by Fiji (thanks to the Bio-Formats plugin.

The easiest method is to mark the files you want to open the folder (in Finder, Explorer, Konqueror or whatever the program is called on your operating system), and drag-n-drop them onto the Fiji main window.

How to navigate through 3D or 4D data

(turning, playing/making movies, projections)

How to measure angles (in 2D or 3D), distances, areas, intensities

How to analyze colocalization of labels

How to stitch together two images

How to view your data using volume rendering

How to segment objects, track them, deconvolution, filtering

all of which 2D, 3D and 4D

How to export measurements for use in other programs, such as R, Matlab or Excel

How to plot graphs from the measurements directly

How to control a microscope

(x-y-z drive, stacks, timeseries, autofocus, find object although they have moved and keep them in the field of view between timepoints; ,maybe even get a warning in the 4th floor, when something fails at the scope in the cellar)

How to make simple macros/scripting (like do a given operation of whole bunch of pictures....)