Difference between revisions of "BigStitcher BrightnessContrastAdjustment"

(Overview)
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Even after correcting for fixed-pattern noise (see [[BigStitcher_Flatfield_correction|here]]), differences in brightness and contrast between images, e.g. due to bleaching, might persist and be visible in the fused images. To correct for this, we estimate optimal linear transforms of pixel intensities in adjacent images to achieve uniform brightness and contrast in the whole dataset. We minimize the intensity difference of all pixels in the overlapping volume of two images according to the current registrations via a linear transform for each image:  
 
Even after correcting for fixed-pattern noise (see [[BigStitcher_Flatfield_correction|here]]), differences in brightness and contrast between images, e.g. due to bleaching, might persist and be visible in the fused images. To correct for this, we estimate optimal linear transforms of pixel intensities in adjacent images to achieve uniform brightness and contrast in the whole dataset. We minimize the intensity difference of all pixels in the overlapping volume of two images according to the current registrations via a linear transform for each image:  
 
<math>I'(x)=I(x)*\alpha+\beta</math>
 
<math>I'(x)=I(x)*\alpha+\beta</math>
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The brightness and contrast adjustment is available in '''Multiview mode''' under {{bc|Processing|Intensity Adjustment}}.
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{{Notice|Calculating the intensity adjustment requires the images to be aligned, therefore use it as a last step after registration before fusing the dataset.}}
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=== Usage ===
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==== Displaying ====
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==== Removing ====

Revision as of 09:49, 14 January 2019

Overview

Even after correcting for fixed-pattern noise (see here), differences in brightness and contrast between images, e.g. due to bleaching, might persist and be visible in the fused images. To correct for this, we estimate optimal linear transforms of pixel intensities in adjacent images to achieve uniform brightness and contrast in the whole dataset. We minimize the intensity difference of all pixels in the overlapping volume of two images according to the current registrations via a linear transform for each image: I'(x)=I(x)*\alpha+\beta

The brightness and contrast adjustment is available in Multiview mode under Processing ▶ Intensity Adjustment.



Usage

Displaying

Removing