Otsu Thresholding

Authors: Christopher Mei (christopher.mei at sophia.inria.fr)
Anthony Joshua (Anthony.Joshua at utoronto.ca)
Tony Collins (tonyc at uhnresearch.ca)
History: 2003/12/15 : First version
2005/06/15: Added 16-bit version
Requires:ImageJ 1.31p or later, which adds the ability to package plugins in JAR files
Source:Contained in Otsu_Thresholding.jar, which can be opened using a ZIP utility
Installation: Download Otsu_Thresholding.jar to the plugins folder, or subfolder, restart ImageJ, and there will be a new Plugins/Filters/Otsu Thresholding... command.
Description: This algorithm is an implementation of the Otsu thresholding technique [otsu79]. The histogram is divided in two classes and the inter-class variance is minimized. This plugin outputs a thresholded image with the selected threshold. 
  
@Article{otsu79,
  author =       "N. Otsu",
  title =        "A threshold selection method from gray level
                 histograms",
  journal =      "{IEEE} Trans. Systems, Man and Cybernetics",
  year =         "1979",
  volume =       "9",
  pages =        "62--66",
  month =        mar,
  keywords =     "threshold selection",
  note =         "minimize inter class variance",
}
A 16-bit version of this plugin (OtsuThresholding_16Bit.java), contributed by Anthony Joshua and Tony Collins, is also available. They also contributed a macro (FullTelomerecounter) that uses the 16-bit version.

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