Thomas Kuo <thekuo[at]ece.ucsb.edu>
Jiyun Byun <jiyun[at]ece.ucsb.edu>
ITCN is based on work at the Center for Bio-image Informatics at UC Santa Barbara
Requires: ImageJ 1.34s or later Source: Contained in ITCN_.jar, which can be opened using a ZIP utility Installation: Download ITCN_.jar to the plugins folder or subfolder, restart ImageJ,
and there will be a new Plugins/Filters/ITCN commands.
ITCN is an ImageJ plugin for automatically counting the number cells within an image. The inputs are: (1) an estimation of the diameter of a cell, (2) an estimation of the minimum distance between cells, and (3) either a region of interest (ROI) selected with ImageJ's selection tools or a black and white mask image that is white in regions that are to be counted.
Full instructions are available at www.bioimage.ucsb.edu/itcn.html.
- Use of magnification information to initially estimate width and minimum distance.
- Advanced options including Threshold level and dot/line colors
- Options for generating a mask.
- Automatic determination of cell width.