// Alignment Macros // // Use these macros to align the frames in a time-lapse // stack using simple xy translation. First use the "Add // and Advance" and/or "Add and Go Back" macros to add a // point selection for each frame to the ROI Manager. Then run the // "Align" macro, which uses the Image>Translate command // to align each frame based on the change in the // coordinates of that frame. macro "Add and Advance [a]" { roiManager("add"); run("Next Slice [>]"); } macro "Add and Go Back [b]" { roiManager("add"); run("Previous Slice [<]"); } macro "Align" { roiManager("select", 0); run("Measure"); xref = getResult("X"); yref = getResult("Y"); n = roiManager("count"); for (i=0; i