34 Key Modifiers

File▷Open Next [O]↑ Open previous

File▷Revert [r]↑ Skip dialog prompt

Edit▷Copy [c]↑ Copy to system clipboard

Image▷Color▷Split Channels↑ Keep original image

Image▷Stacks▷Add Slice↑ Insert before current slice

Image▷Stacks▷Next Slice [>]↑ Skip ten slices

Image▷Stacks▷Previous Slice [<]↑ Skip ten slices

Image▷Stacks▷Start Animation [\]↑ Show options dialog

Image▷Duplicate… [D]↑ Skip dialog prompt

Image▷Overlay▷Add Selection… [b]↑ Show options dialog

Process▷Enhance Contrast…↑ Do classic histogram equalization

Analyze▷Histogram [h]↑ Show dialog prompt

Analyze▷Plot Profile [k]↑ For rectangular selections, generate row average plot. For wide straight lines, display rotated contents

Analyze▷Gels▷↑Select First Lane Assumes lanes are horizontal

Analyze▷Tools▷Analyze Line Graph↑ Show intermediate image

Analyze▷Tools▷ROI Manager↑ (Add) Name and add selection

Plugins▷Utilities▷ImageJ Properties…↑ List all Java properties

Area Selection Tools↑ Subtract current selection from the previous one

Rectangular Selection Tool↑ and Oval Selection Tool↑ Current aspect ratio is maintained while resizing

Straight Line Selection Tool↑ Keeps the line length fixed while moving either end of the line. Forces the two points that define the line to have integer coordinate values when creating a line on a zoomed image

Segmented Line Selection Tool↑ and Polygon Selection Tool↑ Alt-clicking on a node deletes it

Point Tool↑ Alt-clicking on a point deletes it

Color Picker Tool↑ Alt-clicking on an image ‘picks-up’ background color

All Tools↑ Show location and size in pixels rather than calibrated units

Image▷Adjust▷Threshold… [T]↑ Adjusting Min also adjusts Max

Image▷Adjust▷Brightness/Contrast… [C]↑ Apply adjustments to all channels of a composite image

Installed Macros↑ and Scripts↑ Open instead of run

Rectangular Selection Tool↑ and Oval Selection Tool↑ Forces 1:1 aspect ratio

Area Selection Tools↑ Add selection to previous one

Polygon Selection Tool↑ Shift-clicking on a node duplicates it

Straight Line Selection Tool↑ Forces line to be horizontal or vertical

Segmented Line Selection Tool↑ Shift-clicking on a node duplicates it

Point Tool↑ Shift-clicking adds points (Multi-point Tool↑ behavior)

Magnifying Glass↑ Shift-clicking and dragging runs Image▷Zoom▷To Selection↑

Rectangular Selection Tool↑ and Oval Selection Tool↑ Selection is resized around its center

Straight Line Selection Tool↑ Line is rotated/resized around its center

Any Tool Switch to the Scrolling Tool↑

Moving Selections↑ The four arrow keys move selection outlines one pixel at a time

Resizing Selections↑ Rectangular and oval selections are resized by holding Alt while using the arrow keys

Stacks↑ Navigation The  ←  and  →  keys substitute for < and > for moving through a stack. If there is a selection, you must also hold Shift

Hyperstacks↑ Navigation The  ←  and  →  keys change the channel. Hold Ctrl to move through the slices and Alt to move through the frames

Zooming The ↑ and ↓ keys zoom the image in and out. If there is a selection, you must also hold either Shift or Ctrl