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Image Intensity Processing

29 bytes removed, 13:27, 19 December 2013
FFT background correction
=== FFT background correction ===
We sometimes see You can correct for uneven illumination and also horizontal "scan lines" in transmitted light images acquired with using confocal microscopes. This background can be corrected by using the native FFT bandpass function (''Process/FFT/Bandpass Filter...).''
Experiment You can experiment with the settings to optimize the filtering. The user can and also choose to filter structures down to a certain number of pixels. The default value is 40 pixels. You can filter small structures up to a certain value. The default value is 3 pixels. The user can choose from a drop down menu whether to suppress stripes with None, Horizontal, or Vertical. The tolerance of direction can be chosen. The default is 5%. Finally, the user can choose whether to allow autoscale after filtering, saturation of the image when autoscaling, whether or not to display the filter, and whether or not to process an entire stack.