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Image Intensity Processing

176 bytes removed, 13:36, 18 December 2013
== Pseudo-linescan ==
“Linescanning” is a mode of acquisition common to many confocal microscopes where Linescanning involves acquiring a single line, one pixel wide line is acquired over in width, from a period of time common confocal microscope instead of the norma1 2-D, x-y a standard 2D image. Usually this allows This is usually a faster acquisitionway to take an image. The All the single pixel -wide images over the time course are then stacked from left to right to generate a 2-D recreate the 2D image (i,e, ''x''-''t'').
A “pseudopseudo-linescan” is the linescan generation of a linescan-type x-t plot from a 3-D (''x, y, t'') timecourse and can be image. It is useful in for displaying 3-D data (x, y, t) in 2 dimensions.
A line of interest must be is drawn followed by the command: “''Image/Stacks/Reslice”'' or with the keyboard button “/”. It will prompt ask you for the line width. Enter the width of line that you wish to be averaged. A It will generate a pseudo-linescan “stack” will be generated, with each slice representing the pseudo-linescan of a single-pixel wide line along the line of interest. To average Average the pseudo-linescan “stack”, select by selecting “''Image/Stacks/Z-Project…''” and select use the ''Average'' command. A polyline poly-line can be used although utilized, but this will only allow generate a single pixel slice to be made.
This example shows the elementary calcium events preceding a calcium wave. HeLa cell loaded with the calcium-sensitive fluorophore, Fluo-3 and imaged whilst responding to application of histamine. ('''A'''). Frame taken from time-course at the peak of the calcium-release response. ('''B''') The line of pixels along X-Y was taken and stacked side by side from right-to left to generate a "pseudo-line scan". This allows visualisation of the progression of the wave from its initiation site.