== Pseudo-linescan ==
“pseudo- linescan” is the generation of a linescan-type x-t plot from a 3-D (''x, y, t'') timecourse and can be useful in displaying 3-D data (x, y, t) in 2 dimensions.
A line of interest
must be drawn followed by the command: “''Image/Stacks/Reslice”'' or keyboard “/”. It will prompt for the line width . Enter the width of line you wish to be averaged. A pseudo-linescan “stack” will be generated, each slice representing the pseudo-linescan of a single-pixel wide line along the line of interest. To average the pseudo-linescan “stack” , select “''Image/Stacks/Z-Project…''” and select the ''Average'' command. A polyline can be used although this will only allow a single pixel slice to be made.
This example shows the elementary calcium events preceding a calcium wave. HeLa cell loaded with the calcium-sensitive fluorophore, Fluo-3 and imaged whilst responding to application of histamine. ('''A'''). Frame taken from time-course at the peak of the calcium-release response. ('''B''') The line of pixels along X-Y was taken and stacked side by side from right-to left to generate a "pseudo-line scan". This allows visualisation of the progression of the wave from its initiation site.