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The implemented least-squares method automatically approximates the solution of the mathematical overdetermined system, so to define the registration matrix then used for aligning the different images. It also considers rotations and scale changes in case of object dilation/shrink. Finally, it provides an iterative subroutine for a fine alignment, to easily reach a very good image co-registration quality.
DS4H Image Alignment
has been implemented in Java as a plugin for ImageJ/Fiji. It works with “. svs” files, but also all the medical imaging formats included in the [[Bio- formats]] library.