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== Pre-processing ==
This section discusses some aspects that should be taken into account when segmenting neuronal arbors to be processed by ''Sholl Analysis''. Since ''image segmentation'' (i.e., the partitioning of images into analyzable parts) is vulnerable to noise and background fluorescence, it is not possible to generalize universal routines that efficiently binarize grayscale images. This means that any procedure that tries to appropriately describe the original fluorescence image with a binary mask must be tailored to the characteristics of individual datasets. As mentioned in [[#Complementary_Tools|Complementary Tools]], several routines listed here as distributed through the [[BAR]]
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