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=== <big>Overview</big> ===
[[File:02b_Hotaling_Visual_Abstract.png|500px|thumb|right|alt=Alt|Overview of DiameterJ analysis flow - (''Top'') SEM image --> segmented image --> stylized Euclidean distance transform. (''Bottom'') A few of the graphs capable of being produced from data given by DiameterJ]] DiameterJ<ref name="Hotaling" />
is a two step process of image analysis:
Image Segmentation into a binary image (black and white pixels only)#* Sixteen default segmentation algorithms have been included with DiameterJ in the "Segment SRM" and "Segment Mixed" plugins. However, these algorithms may not work for all SEM images. #* If the user is not happy with the results of the segmentation algorithms (i.e. the black and white images do not produce an accurate representation of the original image) then DiameterJ will still work with any binary image that has been segmented through some other means.# Analysis of Segmented image#* All measures given by DiameterJ are in pixels by default#* DiameterJ has been validated with over 130 digital images created ''in silico'' and with scanning electron microscope images of reference wires with known diameters. #*:- <b> Fibers that are smaller than 10px or greater than 10% of the smallest dimension of the image produce 10% or greater error</b>#*:- For now DiameterJ only analyzes .tif, jpeg, png, .bmp, and . gif files#*:- Fibers must be less than 512px in diameter to be analyzed
: Hotaling NA, Bharti K, Kriel H, Simon Jr. CG. DiameterJ: A validated open source nanofiber diameter measurement tool. Biomaterials 2015;61:327–38. doi:10.1016/j.biomaterials.2015.05.015.
Download Link ===: For ImageJ 1. 48 or newer: [http://imagej.net/_images/c/cc/DiameterJ_1-018.zip DiameterJ v. 1.018 for ImageJ]: For Fiji latest release: [http://imagej.net/_images/6/65/DiameterJ_Fiji_-_1-018.zip DiameterJ v. 1.018 for Fiji]
=== <big>How DiameterJ Works</big> ===