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Simple ImageJ/Fiji plugin to plot intensity of a spot over time.
To install this plugin check the "ValelabUtils" update site in the Fiji updater.
This plugin has two phases, in the first phase it detects spots. It does so by scanning for local maxima in the image with a box of user-defined size ("Spot radius (pixels)"). Local maxima are accepted when the maximum is higher than a user-defined number ("Noise tolerance") over the average of the 4 corners of the box. An image constructed by averaging the first n images ("Check First n Frames") is used for the spot finding. This same image is used to calculate the background (which is currently done using the ImageJ Background tool using a ball radius of 100 and "Sliding paraboloid" checked).