Skip to content, Skip to search



3 bytes removed, 06:46, 31 March 2018
no edit summary
* In the image field specify the path to a time lapse beads image stored in a format readable by [[Bio-Formats]].
** All result files generated by NoiSee will be placed in a new sub-folder located in the same directory as the input image.
* The approximate beads diameter has to be specified in pixels. NoiSee uses the "''Find Maxima''" function to automatically detect beads above a certain intensity threshold that needs to be roughly estimated. Starting values for this estimate depending depend on the detector type used, e.g. 50 for PMTs, 10 for Hybrid detectors in photon counting mode or 500 for camera based systems.
** If not all beads are identified the estimate should be lower.
** If peaks other than beads are identified the estimate should be higher.