add note: no object based methods here.
Coloc 2 is Fiji's plugin for colocalization analysis. It implements and performs the pixel intensity correlation over space methods of [[wikipedia:Pearson product-moment correlation coefficient|Pearson]], [[Media:Manders.pdf|Manders]], [[Media:Costes etalColoc.pdf|Costes]], [[Media:LietAlColoc.pdf|Li]] and more, for scatterplots, analysis, automatic thresholding and statistical significance testing.
<span style="color:red">There are many nuances and pitfalls to colocalization analysis. As such, we strongly recommend you read the [[Colocalization Analysis]] section of the [[Cookbook]] before attempting to use Coloc 2!</span>