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Colocalization Analysis

3 bytes added, 14:18, 30 July 2015
Why scatter plots instead of colour merge images?: change to red green yellow spirals image, as thats what the macro makes these days
Far too often, composite/merge images of red and green channels are considered sufficient to demonstrate colocalisation. This is plain wrong. The problems with red/green merge images for colour-blind people aside, there is another very good reason to require scatter plots: the perception of human eyes and brain can be fooled very easily. Just have a look at this image:
[[Image:SpiralsSpiralsRGY.png|300px|center]]
Most people might think that the prominent spirals are in shades of green and blue, but they actually have <b>exactly the same color!</b> You can verify this yourself by calling File>Open Samples>[[Spirals (Macro)]] in Fiji.