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Colocalization Analysis

60 bytes added, 08:48, 9 July 2015
qualify uselessness of % colocalisation measures, to only useless without use of a biologically relelvant region of interrest
The % volume and intensity colocalised values are pretty useless , unless you use a biologically relevant region of interest, as they are very dependent on how much stuff and how much background there is in a certain image, and you will get totally different values for different images of the same sample, that happen to have different areas of background.
In images that have no empty regions, like tissue samples, it might then be a useful number. Maybe % intensity above threshold colocalised might be a useful value in some cases?