<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div>Hi all, </div><div><br></div><div style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;">I need to get the second channel (green) image after running "Split Channels" command in imageJ menu:</div><div style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;"><br></div><div style="background-color: transparent;"> IJ.run(imp,"Split Channels","");<br></div><div style="background-color: transparent; color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; font-style: normal;"><br></div><div style="background-color: transparent; color: rgb(0, 0, 0); font-size: 16px; font-family: 'times
new roman', 'new york', times, serif; font-style: normal;">when using "IJ.getImage()" I got the last image which is the third channel (blue).</div><div style="background-color: transparent; color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; font-style: normal;"><br></div><div style="background-color: transparent; color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; font-style: normal;">any help please?</div><div style="background-color: transparent; color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; font-style: normal;"><br></div></div></body></html>