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<b><a class="bz_bug_link
bz_status_NEW "
title="NEW - Wrong metadata for Leica lif files"
href="http://fiji.sc/bugzilla/show_bug.cgi?id=1173#c1">Comment # 1</a>
on <a class="bz_bug_link
bz_status_NEW "
title="NEW - Wrong metadata for Leica lif files"
href="http://fiji.sc/bugzilla/show_bug.cgi?id=1173">bug 1173</a>
from <span class="vcard"><a class="email" href="mailto:lingfengtang1982@gmail.com" title="lingfengtang1982@gmail.com">lingfengtang1982@gmail.com</a>
</span></b>
<pre>Created <span class=""><a href="attachment.cgi?id=267" name="attach_267" title="example lif file">attachment 267</a> <a href="attachment.cgi?id=267&action=edit" title="example lif file">[details]</a></span>
example lif file
In case you cannot see the right metadata, here it is:
Image: ppkR-mCard-40x CB z4 633 0.5p
Size: 524.29 KB
File Location: D:\02C盘\桌面\Experiment001.lif
Start Time: 9/27/2015 12:06:23 AM.620
End Time: 9/27/2015 12:06:23 AM.620
Total Exposures: 2 (2 channels, 1 frames)
Data from: LAS-AF 3.3.0.10134
________________________________________
Dimensions
Dimension Logical Size Physical Length Physical Origin Pixel Size /
Voxel Size
X 512 72.66 µm 0.00 µm 0.142 µm
Y 512 72.66 µm 0.00 µm 0.142 µm
Channels
LUT Resolution Min Max STED: DetectorMode / Huygens saturation
factor / Wavelength
Red 8 0 255 --- / --- / ---
Green 8 0 255 --- / --- / ---
Time Stamps:
Frame (Show All)
Relative Time (s) Absolute Time (h:m:s.ms) Date
1 0 12:06:23 AM.620 9/27/2015
2 0 12:06:23 AM.620 9/27/2015
Frame (Show first + last)
Relative Time Absolute Time Date
1 0 12:06:23 AM.620 9/27/2015
2 0 12:06:23 AM.620 9/27/2015
Confocal Settings
Name Value
Scan Mode xyz
Scan Direction X Bidirectional
Scan Speed 600 Hz
Version Number 6
StagePosX 15205.0 µm
StagePosY 7973.1 µm
ZPosition 9.2 µm
IsSuperZ 0
Magnification 40
ObjectiveName HC PL APO CS2 40x/1.30 OIL
Immersion OIL
NumericalAperture 1.30
RefractionIndex 1.518
Zoom 4.00
Pinhole 130.6 µm
PinholeAiry 2.00 AU
EmissionWavelength for PinholeAiry Calculation 580.0 nm
FrameAverage 1
LineAverage 2
FrameAccumulation 1
Line_Accumulation 1
IsUserSettingNameSet 0
IsRoiScanEnable 0
Filter Wheels / Other Motorized Devices
Device Name Filter Name/Position
Excitation Beam Splitter TD 488/561/633
Galvo Slider Galvo X Normal
Notch FW 2 Empty
Polarization FW NF 488/561/633
Galvo Resonant Pan Galvo X Pan Center
Simple Beam Expander No FRAP Booster
Target Slider Target Park
X2 Lens Changer CS2 UV Optics 1
Laser Lines
Laser Line Intensity
UV ( 405 nm) Shutter: off, Intensity: 0.0000%
Visible ( 458 nm) Shutter: on, Intensity: 0.0000%
Visible ( 488 nm) Shutter: on, Intensity: 0.0000%
Visible ( 514 nm) Shutter: on, Intensity: 0.0000%
Visible ( 561 nm) Shutter: on, Intensity: 0.1001%
Visible ( 633 nm) Shutter: on, Intensity: 0.0000%
Detectors
Name Channel Type Location Active Gain Offset Gate
Start Gate End Gate Ref. Wavelength
PMT 1 Channel 1 PMT (380nm - 391nm) Internal Inactive 0.0
0.00 -- Time Gating not supported --
HyD 2 Channel 2 HyD (566nm - 601nm) Standard mode Internal
Inactive 15.0 -0.01 -- Time Gating not supported --
PMT 3 Channel 3 PMT (621nm - 626nm) Internal Inactive 0.0
0.00 -- Time Gating not supported --
HyD 4 Channel 4 HyD (640nm - 700nm) Standard mode Internal Active
50.0 -0.01 -- Time Gating not supported --
PMT 5 Channel 5 PMT (737nm - 779nm) Internal Inactive 0.0
0.00 -- Time Gating not supported --
PMT Trans Transmission Channel PMT TLD Inactive 0.0 0.00
-- Time Gating not supported --
Lasers
LaserName OutputPower
405 Diode Off
Argon 0 %
DPSS 561 On
HeNe 633 On
Sequential Setting Nr.1
PMT Inactive, Gain: 0, Offset: 0
HyD Active, Gain: 0, Offset: 0
PMT Inactive, Gain: 0, Offset: 0
HyD Inactive, Gain: 0, Offset: 0
PMT Inactive, Gain: 0, Offset: 0
PMT Inactive, Gain: 0, Offset: 0
Laser Line UV ( 405 nm) Intensity: 0.0000%
Laser Line Visible ( 458 nm) Intensity: 0.0000%
Laser Line Visible ( 488 nm) Intensity: 0.0000%
Laser Line Visible ( 514 nm) Intensity: 0.0000%
Laser Line Visible ( 561 nm) Intensity: 0.1001%
Laser Line Visible ( 633 nm) Intensity: 0.0000%
Sequential Setting Nr.2
PMT Inactive, Gain: 0, Offset: 0
HyD Inactive, Gain: 0, Offset: 0
PMT Inactive, Gain: 0, Offset: 0
HyD Active, Gain: 0, Offset: 0
PMT Inactive, Gain: 0, Offset: 0
PMT Inactive, Gain: 0, Offset: 0
Laser Line UV ( 405 nm) Intensity: 0.0000%
Laser Line Visible ( 458 nm) Intensity: 0.0000%
Laser Line Visible ( 488 nm) Intensity: 0.0000%
Laser Line Visible ( 514 nm) Intensity: 0.0000%
Laser Line Visible ( 561 nm) Intensity: 0.0000%
Laser Line Visible ( 633 nm) Intensity: 0.4999%</pre>
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